Association of Osteopontin (OPN) Level in Diabetic Nephropathy Patients as an Early Diagnostic Marker

Abstract

OBJECTIVES

The study aimed to find an association between OPN levels and diabetic nephropathy as a serum marker in DM patients for early detection.

METHODOLOGY

A cross-sectional study was conducted for 6 months on 277 diagnosed cases of DM with 10 positive and 10 negative controls collected through non-probability sampling. Fasting blood sugar, HbA1c, serum urea and creatinine levels of known diabetic patients were measured to confirm their disease status and OPN using an ELISA Kit and compared in DM patients and controls. Statistical analysis was performed using SPSS v22.0. DM patients and negative controls were compared using unpaired one-way ANOVA and least square methods. The p-value of ≤0.05 was deemed statistically significant.

RESULTS
Osteopontin has a significant relation with diabetes duration only. Serum biomarkers of the DM show significant osteopontin levels with 0.00 p values for HBA1C and all biomarkers of Diabetes. The least-square distribution of the different biomarkers with osteopontin level at 95 % confidence interval and standardized coefficients show lower and upper bound and significant levels. Osteopontin levels show 0.29 and 0.38 lower and upper bound at a 95% confidence interval, with a 0.79 considerable level and 0.25 t distribution, with 0.01 beta values for diabetes duration. Blood creatinine level is 0.02 and 0.05 lower and upper bound, with 0.36 significance level for Osteopontin and 0.02 standard error. Osteopontin levels 11ng/dl to 20 ng/dl have been found in 88 cases, while 09 in positive controls and 12 in negative controls. Meanwhile, only two positive controls were found>20 ng/dl osteopontin level.

CONCLUSION

Serum (OPN) was positively correlated in positive controls and low levels in negative control and regular diabetic patients with no nephropathy.