Salivary Biomarker Levels and Periodontal Health Status in Patients with Diabetic and Healthy Lifestyle: A Comparative Study
Keywords:
Diabetic, Periodontal, Salivary Biomarkers, HyperglycemiaAbstract
OBJECTIVES
To evaluate and compare the periodontal health status and salivary biomarker levels in diabetic patients and non-diabetic individuals, thereby determining the association between these factors.
METHODOLOGY
A comparative study included 100 participants divided into two groups: 50 diabetic patients and 50 healthy controls. Periodontal parameters such as the gingival index (GI), probing pocket depth (PPD), and clinical attachment level (CAL) were assessed. Salivary biomarkers, including interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and glucose levels, were measured using enzyme-linked immunosorbent assay (ELISA) techniques. Statistical analysis was conducted using SPSS software.
RESULTS
Diabetic patients exhibited significantly higher periodontal parameters (GI, PPD, CAL) compared to healthy controls (p < 0.05). Salivary biomarkers, particularly IL-6 and TNF-α, were markedly elevated in the diabetic group (p < 0.01). There was a strong positive correlation between salivary glucose levels and periodontal parameters (p < 0.001).
CONCLUSION
Diabetic individuals show worse periodontal health and elevated salivary inflammatory biomarkers compared to non-diabetic controls. This suggests a need for closer periodontal monitoring in diabetic patients to mitigate further systemic complications.
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References
International Diabetes Federation (IDF) [Internet]. Brill; doi:10.1163/1570-6664_iyb_sim_org_38965
Graves DT, Kayal RA. Diabetic complications and dysregulated innate immunity. Frontiers in bioscience : a journal and virtual library. 2008 Jan 1;13:1227–39
Ghallab NA. Diagnostic potential and future directions of biomarkers in gingival crevicular fluid and saliva of periodontal diseases: Review of the current evidence. Archives of Oral Biology. 2018 Mar;87:115–24
Lontchi-Yimagou E, Sobngwi E, Matsha TE, Kengne AP. Diabetes Mellitus and Inflammation. Current Diabetes Reports. 2013 Mar 14;13(3):435–44
Preshaw PM, Foster N, Taylor JJ. Cross‐susceptibility between periodontal disease and type 2 diabetes mellitus: an immunobiological perspective. Periodontology 2000. 2007 Sep 10;45(1):138-57
Yamada S. Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology). 2007;49(2):109–10
Spadaccio C, Nenna A, Nappi F, Avtaar Singh S, Sutherland F, Di Domenico F, et al. Pharmacologic approaches against Advanced Glycation End Products (AGEs) in diabetic cardiovascular disease. Research in Cardiovascular Medicine. 2015;4(2):5
Krikun E. Diode laser in the inflammatory periodontal diseases complex treatment [Internet]. Morressier; 2018. Doi:10.26226/morressier.5ac383282afeeb00097a43cf
Hajishengallis G, Lamont RJ, Koo H. Oral polymicrobial communities: Assembly, function, and impact on diseases. Cell host & microbe. 2023 Apr 12;31(4):528–38
Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: Consensus report of workgroup 1 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. British Dental Journal. 2018 Jul;225(2):141–141
Preshaw PM, Alba AL, Herrera D, Jepsen S, Konstantinidis A, Makrilakis K, et al. Periodontitis and diabetes: a two-way relationship. Diabetologia. 2012 Jan;55(1):21–31.
Lin X, Yang T, Zhang X, Wei W. Lifestyle intervention to prevent gestational diabetes mellitus and adverse maternal outcomes among pregnant women at high risk for gestational diabetes mellitus. The Journal of international medical research. 2020 Dec;48(12):300060520979130
Jurysta C, Bulur N, Oguzhan B, Satman I, Yilmaz TM, Malaisse WJ, et al. Salivary glucose concentration and excretion in normal and diabetic subjects. Journal of biomedicine & biotechnology. 2009;2009:430426–430426
Clinical Oral Findings and Salivary Analysis of Patients with and Without Diabetes Mellitus. Journal of Oral Biology. 2019 Dec 30;6(2):1-6
Puttaswamy KA, Puttabudhi JH, Raju S. Correlation between Salivary Glucose and Blood Glucose and the Implications of Salivary Factors on the Oral Health Status in Type 2 Diabetes Mellitus Patients. Journal of International Society of Preventive & Community Dentistry. 2017/Jan-Feb;7(1):28-33
Review for “Lactobacillus fermentum attenuates the alveolar bone loss in ligature‐induced periodontitis in mice”. Wiley; 2023.doi:10.1111/odi.14739/v2/review1
Hughes F. Investigation of the effects of periodontal probing on concentrations of salivary biomarkers of Periodontal Disease. Morressier; 2018.doi:10.26226/morressier.5ac383192afeeb00097a427c
Rehman K, Munawar SM, Akash MSH, Buabeid MA, Chohan TA, Tariq M, et al. Hesperidin improves insulin resistance via down-regulation of inflammatory responses: Biochemical analysis and in silico validation. PloS one. 2020 Jan 13;15(1):e0227637–e0227637
Ghallab NA. Diagnostic potential and future directions of biomarkers in gingival crevicular fluid and saliva of periodontal diseases: Review of the current evidence. Archives of Oral Biology. 2018 Mar;87:115–24
Dhir S, Kumar V. Inflammation - The Connecting Bridge between Periodontitis (Gum Disease) and Atherosclerotic Cardiovascular Disease (ACVD). Inflammation - The Connecting Bridge between Periodontitis (Gum Disease) and Atherosclerotic Cardiovascular Disease (ACVD). 2024 Mar 15;I(1):56–9
Mohammad C. Effect of Periodontal Therapy on Salivary Total Protein, TNF-alpha and IL-1 beta in Chronic Periodontitis Patients. Sulaimani Dental Journal. 2018 Aug 31;5(1):9
Gupta S, Nayak MT, Sunitha JD, Dawar G, Sinha N, Rallan NS. Correlation of salivary glucose level with blood glucose level in diabetes mellitus. Journal of oral and maxillofacial pathology : JOMFP. 2017/Sep-Dec;21(3):334–9
Clinical Oral Findings and Salivary Analysis of Patients with and Without Diabetes Mellitus. Journal of Oral Biology. 2019 Dec 30;6(2):1–6.
Singh M, Bains VK, Jhingran R, Srivastava R, Madan R, Maurya SC, et al. Prevalence of Periodontal Disease in Type 2 Diabetes Mellitus Patients: A Cross-sectional Study. Contemporary clinical dentistry. 2019/Apr-Jun;10(2):349–57
Review for “Early markers of periodontal disease and altered oral microbiota are associated with glycemic control in children with type 1 diabetes”. Hindawi Limited; 2020.doi:10.1111/pedi.13170/v1/review1
Wankhede AN, Dhadse PV. Interleukin-17 levels in gingival crevicular fluid of aggressive periodontitis and chronic periodontitis patients. Journal of Indian Society of Periodontology. 2022/Nov-Dec;26(6):552–6
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Copyright (c) 2024 Farooq Maqsood, Huma Abid, Nausheen Daud, Naila Noreen, Faizan Bilal Malik, Tariq Ali Khan
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